The CacoGoblet model offers a fast and reliable approach to in vitro anti-inflammatory drug testing. By assessing TEER and paracellular permeability, this assay provides key insights into intestinal inflammation and compound efficacy.
Permeability assays are important for understanding how molecules cross biological barriers, assessing both their ability to cross and the mechanisms involved. While drugs are often absorbed by passive diffusion, some molecules can also be substrates for membrane proteins or transporters.
A study led by scientist Andrew Novak and published last November in the prestigious Journal of Medicinal Chemistry explored a new therapeutic approach targeting malignant cell proliferation.
Explore our comprehensive cytotoxicity assay protocol featuring advanced testing methods with CacoReady kits. Learn about TEER, LY flux, and how to predict drug toxicity effectively.
The permeability assay protocol for Caco-2 cells serves as a cornerstone in understanding and predicting the intestinal absorption of pharmaceutical agents.
In a publication in the Journal of Medicinal Chemistry, a collaborative research led by the scientist Dr. H. Day, in conjunction with a consortium of researchers, explored the development of allosteric inhibitors targeting SH2 domain-containing protein tyrosine phosphatase-2 (SHP2) for cancer therapy.
In preclinical research, the choice of the appropriate well system format – whether it's a transwell, non-transwell, or individual transwell – can significantly impact the outcome of experiments. Selecting the correct plate is crucial to working with permeability, toxicity, drug screening, or other assays.
This research, titled Fragment-Based Discovery of a Series of Allosteric-Binding Site Modulators of β‑Glucocerebrosidase, highlights the potential of fragment-based drug discovery (FBDD) and employs permeability assays with CacoReady and PreadyPort plates.
It is well known that food intake changes luminal conditions in the stomach and the small intestine, modifying drug bioavailability. Food-drug interactions are one of the major challenges for oral-administered drugs, even more so if considering the growing use of food supplements and functional foods.
